Organiser
Get it on Google Play
Get it on Google Play

Nation Matters

Study paves the way for a new approach to fight infections

WebdeskJul 30, 2021, 07:43 AM IST

Study paves the way for a new approach to fight infections

Recruitment of DNA polymerases by PCNA is an essential step during DNA replication, and blocking this step by targeting PCNA by any means can help tackle infections.

  

New Delhi: A new way of handling diseases could soon be in the offing with a team of researchers gaining new insights into the molecular mechanism of living organisms.

 

Proliferating cell nuclear antigen (PCNA) is a protein that plays a significant role in the DNA replication, repair, and recombination processes of all organisms. Recruitment of DNA polymerases by PCNA is an essential step during DNA replication, and blocking this step by targeting PCNA by any means can help tackle infections.

 

Since PCNA is a structural protein, it is widely accepted as a therapeutic target. Interestingly, its structure looks similar across species, though a PCNA of a given species is rarely functional in another. The new study has resolved this problem.

 

The researchers began by elucidating the X-ray crystal structure of the PCNA of a type of red bread mold called Neurospora crassa and compared its structure-function relationship with other already available PCNA studies to understand cross-species incompatibility. They found that two regions, named inter-domain connecting loop (IDCL) and J loop structures, varied significantly among PCNAs.

 

To validate that these structural differences are indeed accountable for functional incompatibility, they generated mutants of Neurospora crassa PCNA mimicking IDCL and J loop structures of PCNA of yeast called Saccharomyces cerevisiae. Genetic analyses suggested that the mutants were fully functional in Saccharomyces cerevisiae. Further, the susceptibility of the strains harbouring ScPCNA mimics of NcPCNA to various genotoxic agents was similar to that in yeast cells expressing ScPCNA. It was clear that structures of the IDCL and J loop of PCNA are critical determinants of interspecies functional compatibility.

 

Speaking to India Science Wire, the scientists said, “Our study has helped us understand the details of the complex interplay of binding between PCNA and its partner proteins in cells.

 

This would help in the development of therapeutic agents that target PCNA. It will be now possible to ensure that the drugs are highly species-specific.”

 

The study was conducted by researchers from the Laboratory of Genomic Instability and Diseases and Laboratory of Macromolecular Crystallography at the Department of Infectious Disease Biology of the Institute of Life Sciences, Bhubaneswar.

 

The team was led by Dr Narottam Acharya of ILS’s Laboratory of Genomic Instability and Diseases and Dr Dileep Vasudevan of ILS’s Laboratory of Macromolecular Crystallography.

 

The other members were Premlata Kumari, Rajiv Gandhi Sundaram, and Kodavati Manohar.

 

They have published a paper on their work in the Journal of Biological Chemistry.

 

Courtesy: India Science Wire

Comments

Also read: DEFENCE SCAN: MULTILATERAL MARITIME EXERCISE MALABAR 2021 – PHASE II ..

Kushal Konwar: The Only Martyr In Last Phase of Quit India Movement

Download Organiser Mobile App:
Mobile App: https://play.google.com/store/apps/de...

Visit Us At:
Website: https://www.organiser.org/

Follow us on:
Facebook: https://www.facebook.com/eOrganiser

Koo: https://www.kooapp.com/profile/eOrganiser

Twitter: https://twitter.com/eOrganiser

Telegram: https://t.me/eorganiser

Instagram: https://www.instagram.com/organiser_weekly

Background Music Credits: https://www.bensound.com

Also read: Researchers Design White Light Emitters for LED Applications ..

J-K: Indian Army organises marathon to commemorate 'Swarnim Vijay Varsh' of 1971 Indo-Pak war, 'Azadi ka Amrit Mahotsav'
The ‘Houses’ of Delhi – Princely Embassies in an Imperial Capital

DEFENCE SCAN: CLOSING CEREMONY: INDO-NEPAL JT EX “SURYA KIRAN XV”

The 15th Edition of Indo-Nepal Joint Training Exercise, SURYA KIRAN XV culminated on October 2, 2021 after 14 days of rigorous training. The exercise was focused on Counter Terrorism and Disaster Relief operations.    INDIA AND US TO SET-UP JOINT WORKING GROUP IN DEFENCE INDUSTRIAL SECURITY The Industrial Security Agreement (ISA) summit between India and the US was held from September 27 to October 1, 2021 at New Delhi. The summit was organised to develop protocol for the ...

DEFENCE SCAN: CLOSING CEREMONY: INDO-NEPAL JT EX “SURYA KIRAN XV”